Multiple Reaction Monitoring (MRM)
نویسندگان
چکیده
منابع مشابه
Data Pre-Processing for Label-Free Multiple Reaction Monitoring (MRM) Experiments
Multiple Reaction Monitoring (MRM) conducted on a triple quadrupole mass spectrometer allows researchers to quantify the expression levels of a set of target proteins. Each protein is often characterized by several unique peptides that can be detected by monitoring predetermined fragment ions, called transitions, for each peptide. Concatenating large numbers of MRM transitions into a single ass...
متن کاملProteomics Multiple Reaction Monitoring ( MRM ) Principles and Application to Coronary Artery Disease Gabriela
Multiple reaction monitoring (MRM) is a mass spectrometry technique based on selection of a peptide ion and one or more characteristic fragment ions. The MRM method has previously been shown to be specific, accurate, and reproducible between laboratories. Through the use of retention time windows, MRM-based quantitation can also be multiplexed to analyze and quantitate hundreds of proteins per ...
متن کاملMRMaid, the web-based tool for designing multiple reaction monitoring (MRM) transitions.
Multiple reaction monitoring (MRM) of peptides uses tandem mass spectrometry to quantify selected proteins of interest, such as those previously identified in differential studies. Using this technique, the specificity of precursor to product transitions is harnessed for quantitative analysis of multiple proteins in a single sample. The design of transitions is critical for the success of MRM e...
متن کاملProteomics Multiple Reaction Monitoring ( MRM ) Principles and Application to Coronary Artery Disease
Multiple reaction monitoring (MRM) is a mass spectrometry technique based on selection of a peptide ion and one or more characteristic fragment ions. The MRM method has previously been shown to be specific, accurate, and reproducible between laboratories. Through the use of retention time windows, MRM-based quantitation can also be multiplexed to analyze and quantitate hundreds of proteins per ...
متن کاملA Multiple Reaction Monitoring (MRM) Method to Detect Bcr-Abl Kinase Activity in CML Using a Peptide Biosensor
The protein kinase Bcr-Abl plays a major role in the pathogenesis of chronic myelogenous leukemia (CML), and is the target of the breakthrough drug imatinib (Gleevec™). While most patients respond well to imatinib, approximately 30% never achieve remission or develop resistance within 1-5 years of starting imatinib treatment. Evidence from clinical studies suggests that achieving at least 50% i...
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ژورنال
عنوان ژورنال: Circulation: Cardiovascular Genetics
سال: 2012
ISSN: 1942-325X,1942-3268
DOI: 10.1161/circgenetics.111.959528